Yes you can. You will need a virtual camera software that connects with your DSLR camera.
Such recommended software is SparkoCam (works with both Nikon and Canon DSLRs) and Ecamm Live:
https://sparkosoft.com/sparkocam (Windows)
https://www.ecamm.com/mac/ecammlive/ (Mac)

After installing SparkoCam and configuring it to capture your DSLR and a microphone, simply select the SparkoCam virtual camera and microphone in Loola:

From the drop-down list, select the SparkoCam video and audio:


Mac Users: Ecamm Live has a virtual camera that works similarly to the SparkoCam virtual camera.

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