Yes you can. You will need a virtual camera software that connects with your DSLR camera.
Such recommended software is SparkoCam (works with both Nikon and Canon DSLRs) and Ecamm Live:
After installing SparkoCam and configuring it to capture your DSLR and a microphone, simply select the SparkoCam virtual camera and microphone in Loola:
From the drop-down list, select the SparkoCam video and audio:
Mac Users: Ecamm Live has a virtual camera that works similarly to the SparkoCam virtual camera.